荣昌猪杂交群体基因组拷贝数变异鉴定与分析

doi:10.11843/j.issn.0366-6964.2014.04.003

Abstract

Abstract:

The aim of this study was to detect CNV regions(CNVRs) from 150 Rongchang pig hybridization population using the Porcine SNP60 BeadChip.In this study，5 CNVRs (CNV regions) were identified across five autosomal chromosomes(chromosomes 1,7,8,13,14) and the predicted status for the CNVRs was 3 for deletion，2 for duplication.Among these 5 CNVRs，CNVR5 and CNVR1 were the novel found states，and CNVR2 and CNVR3 were reported by others but not had been validated.The CNVRs were validated by real time quantitative PCR.The results showed that CNVR2，CNVR3，CNVR5 had extensive polymorphism in different pig breeds.CNVR4 was the false positive states.Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that 46 protein-coding genes in verifying CNVRs possessed a great variety of molecular functions,such as olfactory receptor activity,ethanol metabolic process,neurological system development and fatty acid metabolism pathway.The result provide genetic variation materials for analyzing the genetic base of phenotypic variation between pig breeds.

CLC Number:

S828
S813.3

LONG Xi，QIU Xiao-tian,CHEN Lei,WANG Jin-yong,LI Xue-wei. Identification and Analysis of Copy Number Variations in Rongchang Pig Hybridization Population[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 524-532.

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Identification and Analysis of Copy Number Variations in Rongchang Pig Hybridization Population

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